Indian scientists from ARI identify bacteria capable of methane mitigation in rice plants

Pune: Scientists at the Agharkar Research Institute (ARI), Pune, an autonomous institute under the Department of Science & Technology (DST), have isolated 45 different strains of methanotrophic bacteria which have been found to be capable of reducing methane emissions from rice plants.

Methanotrophs metabolise and convert methane into carbon dioxide. They can effectively reduce methane emission, which is the second most important greenhouse gas (GHG) and 26 times more potent as compared to carbon dioxide. In rice fields, methanotrophs are active near the roots or soil-water interfaces.

Dr Monali Rahalkar, Scientist from the Bioenergy Group, ARI, and her team working on methanotrophs, have enriched, isolated, and cultivated the 45 different strains of methanotrophs and created the first indigenous methanotroph culture. In their work published in Antonie van Leeuwenhoek, an International Journal of General and Molecular Microbiology, they isolated indigenous methanotrophs from Western and Southern India, mainly from rice field soils and freshwater mud, and have documented two novel genera and six novel species of methanotrophs from rice fields in western India. In pot trials, some of the strains were used as bio-inoculants in rice plants.

The team found a decrease in methane emissions from inoculated plants with a positive or neutral effect on the growth of the rice. This could lead to the development of microbial inoculants for methane mitigation in rice.

Rice fields are human-made wetlands and are waterlogged for a considerable period. Anaerobic degradation of organic matter results in the generation of methane. Rice fields contribute to nearly 10% of global methane emissions. Very few studies in the world have focused on methanotrophs from tropical wetlands or tropical rice fields.

Before scientists at ARI commenced their studies, practically no cultures of indigenously isolated methanotrophs from India were available. Native and relevant methanotrophs isolated from rice fields can be excellent models to understand the effects of various factors on methane mitigation. Ammonium fertilizers and increasing temperatures (due to global warming) are some of the important factors which the team plans to study in the future.

Various methanotrophs on culture media (upper left);
The dark pink color biomass from methanotrophs which is a rich source of carotenoids, single-cell proteins leading to methane valorization (upper right); Preliminary experiments indicated that when methanotrophs were inoculated rice plants showed lower methane emissions, higher methane oxidation potential and better/ neutral effect on plant growth (lower left);
Plastic chamber to measure methane emissions is shown (lower right).

Besides methane mitigation studies, methanotrophs can also be used in methane value addition (valorization) studies. Bio-methane generated from waste can be used by the methanotrophs and can be converted to value-added products such as single-cell proteins, carotenoids, biodiesel, and so on. The team is further working on methane valorization studies from the isolated methanotrophs. Such studies help reduce GHG emissions, especially anthropogenic or man-made emissions, which is a pressing need in the age of global warming.


This information was provided by the Communications Team at the Dept. of Science & Technology, Ministry of Science & Technology.


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