Pune: The biofuel sector could get a boost, with researchers at the International Centre for Genetic Engineering and Biotechnology (ICGEB) developing a method to improve the growth rate and sugar content of a marine microorganism called Synechococcus sp. PCC 7002.
Most biotechnological processes, including biofuel production, are dependent on the availability of low-cost and sustainable supply of sugars and a nitrogen source. The sugars typically come from plants. Plants utilize light energy through the process of photosynthesis to convert carbon dioxide in the atmosphere into biological components such as sugars, proteins and lipids.
However, some bacteria, such as the cyanobacteria, also known as blue-green algae, too can perform photosynthesis and produce sugar by fixing the carbon dioxide in the atmosphere. The yield of sugars from cyanobacteria could potentially be much higher than that of land-based crops. Further, unlike plant-based sugars, cyanobacterial biomass provides a nitrogen source in the form of proteins.
Cyanobacteria are found in marine as well as freshwater. Using marine cyanobacteria could be better as freshwater is increasingly getting scarce. However, there is a need to significantly improve their growth rates and sugar content in order to improve the economic feasibility of marine cyanobacteria-based sugar production. A team from ICGEB has achieved this.
Shireesh Srivastava, Group Leader of the System Biology for Biofuel Group of the Centre and an investigator in the Department of Biotechnology (DBT)-ICGEB Centre for Advanced Bioenergy Research, and Jai Kumar Gupta, a PhD student at ICGEB led the team. They have successfully engineered a marine cyanobacterium called Synechococcus sp. PCC 7002 which showed a higher growth rate and sugar (glycogen) content. When grown on air, the growth was doubled and the glycogen content of the cells increased by about 50%.
Srivastava noted that Synechococcus sp. PCC 7002 is a model marine cyanobacterium and there were other Synechococcus species or related organisms where this work can be extended right away.
“We are conducting several follow-up studies related to this work including scaling up the cultures to larger volumes, growing cells on urea from human and animal urine, optimizing the extraction of sugars and proteins from the cyanobacterial biomass, and a proof-of-concept production of a biotechnological product such as bioethanol from the processed biomass,” he added.
The Department of Biotechnology had sponsored the research. The scientists have published a report on their work in the journal, ‘Biotechnology for Biofuels’.